Journal: International Journal of Molecular Sciences
Article Title: Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart
doi: 10.3390/ijms22010109
Figure Lengend Snippet: Effect of SCD1 deficiency and hypothyroidism on lipolysis in cardiomyocytes. Protein levels of adipose triglyceride lipase (ATGL), α/β-hydrolase domain containing 5 (ABDH5), G 0 /G 1 switch protein 2 (G0S2), hormone-sensitive lipase (HSL), and phosphorylated HSL (pHSL) at Ser563 and Ser565 in cardiomyocytes in ( A ) wildtype (WT) and ( B ) SCD1 −/− mice were determined by Western blot. mRNA levels of Pnpla2 , Abdh5 , and Lipe were determined in the heart in WT and SCD1 −/− mice by real-time polymerase chain reaction. The data are representative of n = 5 animals/group. The data are expressed as mean ± SD. * p < 0.05, vs. WT; & p < 0.05, vs. SCD1 −/− .
Article Snippet: ABDH5 (36A, sc-100468), AMPK (H-300, sc-25792), CD36 (sc-9154), DGAT1 (sc-271934), DGAT2 (4C1, sc-293211), FAS (H-300, sc-20140), G0S2 (G-12, sc-133423), GPAT1 (D-10, sc-398135), HSL (G-7, sc-74489), PPARα (H-2, sc-398394), SCD1 (E-15, sc-14720), SREBP1 (2A4, sc-13551), and β-actin (C4, sc-47778) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Techniques: Western Blot, Real-time Polymerase Chain Reaction